Research - Grants
Assessment of the Human Papillomavirus Diversity in Cervical Dysplasia Patients in the Czech Republic Using Consensus Primer Polymerase Chain Reaction
Period: 1994-1996
Grant agency: GAČR 312/94/0886
Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Associate Investigator: Prof. MUDr. Vladimír Vonka DrSc, Institution of hematology and blood transfusion
Keywords: carcinoma, PCR, papillomaviruses
Study of pathogenesis of cervical intraepithelial neoplasias caused by human papillomaviruses
Period: 1997-1999
Grant agency: IGA MZ 4070
Investigator: RNDr. Eva Hamšíková, Institution of hematology and blood transfusion
Associate Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Keywords: papillomavirus, pre-cancer lesions, antibodies, DNA
Role of HPV in progression of atypias of undetermined significance, squamous or glandular (ASCUS/AGUS)
Period: 2000-2002
Grant agency: IGA MZ NC5959
Investigator: RNDr. Eva Hamšíková, Institution of hematology and blood transfusion
Keywords: papillomaviruses, cervical cancer, ASCUS/AGUS, HPV DNA, PCR, Hybrid Capture II, anti HPV antibodies, VLP
Annotation: The association of manifestation of high-risk genital HPV infection (HPV DNA ,anti HPV antibodies) and progression of borderline cytological findings ASCUS/AGUS The comparison of sensitivity and specificity of HPV DNA by PCR and Hybrid Capture II in cervical smears: evaluation of Hybrid Capture II system in management of women with ASCUS/AGUS findings
Role of human papillomaviruses in the origin of malignant and non-malignant diseases of the male urogenital tract
Period: 2000-2002
Grant agency: IGA MZ NI6077
Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Associate Investigator: Doc. MUDr. Michael Urban , Charles University 3rd Faculty of Medicine
Keywords: urogenital, HPV, reservoir, malignant, lesion
Annotation: To identify the reservoir of HPV in male urogenital tract. To reveal the role of HPV in the origin of malignant and non-malignant diseases of the male urogenital tract. The sampling will be done during the open surgery to eliminate the risk of contamination
Epidemiology of TTV in the Czech republic
Period: 2000-2003
Grant agency: GAČR 310/00/1060
Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Associate Investigator: RNDr. Vratislav Němeček CSc., National institute of public health
Keywords: TTV, PCR, hepatitis, transmission
Annotation: Even with the present viral screening procedures to assure the safety of blood and blood products, there is still arisk of acute and chronic posttransfusion hepatitis with unknown ethiology. In 1997, a novel virus (TTV: transfusion transmitted virus) has been isolated. TTV has been detected in 12-62% of blood donors, and a higher prevalence of TTV was found in hemophilia-,hemodialysis- and beta-thalassemic patients. A mainly parental route of transmission had therefore been suggested, although other ways of transmission can at present not be excluded. Some studies seem to indicate that TTV could be associated with some cases of virus-induced hepatitis. Since there are no data yet concerning the prevalence of TTV in the Czech Republic, it is importan to establish laboratory methods for the detection of TTV. This will allow to explore the prevalence of TTV in blood donors and in groups with a high risk of parental or sexual infection.
Determination of the potential risk of human papillomavirus polymorphisms in cervical cancor development
Period: 2000-2001
Grant agency: NATO Collaborative Linkage Grant agency F818
Investigator: Dr. Massimo Tommasino, Deutsches Krebsforschungszentrum, Angewandte Tumorvirologie, Heidelberg, Germany
Associate Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Keywords: E6, E7, polymorphism, papillomavirus, cervical intraepithelial neoplasia
Annotation: Infection with high-risk human papillomavirus (HPV) is necessary for the development of a cervical lesion, but only a fraction of precursor lesions progress to cancer. Additional factors, other than HPV type per se, are likely to increase the probability for progression. Intratype genome variations of HPV have been reported to be associated with viral persistence and the development of a major cervical disease. The prevalence of specific HPV16-E6 variants varies among the patients and depends on stupy population. The data indicate that host genetic factors, such as HLA polymorphism, may determine the potential oncogenicity of the HPV16-E6.
Role of HPV in aetiology of oral cavity/ oropharyngeal cancer
Period: 2001-2003
Grant agency: IGA MZ NC6441
Investigator: Doc. MUDr. Jan Klozar CSc., The Motol universisty hospital
Associate Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Keywords: oropharynx, oral cavity, tumors, HPV, PCR, DNA hybridization, risk factors
Annotation: To detect prevalence and type of HPV in the tissue removed from oropharyngeal/oral carcinoma by PCR,DNA hybridization and sequencing.To detect in the same group of patients and in the group of controls presence and type of HPV in cytology material. To determine whether presence and type of HPV is an independent risk factor of oropharyngeal/oral cancer.To determine whether some other factors (kife style and habits) are also an independent risk factors of oropharyngeal/oral cancer. To determine the presence of HPV antibodies in cancer patients and in healthy controls. To follow-up the antibody level in relation to clinical outcome.To find out whether HPV antibodies might serve as a marker for oropharyngeal/oral cancer risk or for its recurrence.
HPV and other risk factors of oral cavity/ oropharyngeal cancer
Period: 2001-2004
Grant agency: NIH FIRCA 1R03TW001500-01.
Investigator: Prof. Elaine Smith, College of Public Health, University of Iowa, Iowa City, USA
Associate Investigator: RNDr. Ruth Tachezy Ph.D., Institution of hematology and blood transfusion
Keywords: oropharynx, oral cavity, tumors, HPV, PCR, DNA hybridization, risk factors
Annotation: To determine the prevalence and types of HPV DNA in histology speciment of oropharyngeal and oral cavity cancer and to determine, whether HPV DNA positive and negative cases differ in epidemiological and clinical features such as gender, age, tabaco, location and extent of tumor.
Role of HPV in anogenital tumors
Period: 2003-2005
Grant agency: IGA MZ NC7548
Investigator: RNDr. Eva Hamšíková, Institution of hematology and blood transfusion
Associate Investigator: Doc. MUDr. Ladislav Horák DrSc., Charles University 3rd Faculty of Medicine
Keywords: HPV infection, vulvar cancer, anal cancer, PCR, HPV DNA, HPV antibodies, HPV typing
Annotation: To ascertain the role of HPV infection in vulvar squamous cell carcinoma type I and type II development by means of PCR detection of HPV DNA in biopsies of cancer and preceding lesions and assignment of HPV antibodies in patient sera To ascertain the rol e of HPV infection in anal squamous cell carcinoma development by means of PCR detection of HPV DNA in biopsies of cancer and preceding lesions and ssignment of HPV antibodies in patient sera To compare the prevalence of HPV specifixc antibodies in sera from cases and matched controls, compare HPV DNA type found with antibody specificity.
HPV vaccine – prevaccination study
Period: 2006-2008
Grant agency: IGA MZ NC7548
Investigator: RNDr. Eva Hamšíková, Institution of hematology and blood transfusion
Associate Investigator: Doc. MUDr. Lukáš Rob CSc., The Motol universisty hospital
Keywords: HPV infection, HPV vaccines, HPV DNA, HPV antibodies, RLB, virus-like particles
Annotation: To determine the prevalence of HPV types in severe genital and head and neck HPV associated lessions preceding routine HPV vaccination. To determine the prevalence of HPV specific antibodies in sera of individuals collected for seroepidemiological surveys, healthy blood donors and patients with HPV-associated lesions preceding routine HPV vaccination
Prevalence of antibodies to TT virus in the general population of the Czech Republic
Period: 2006-2008
Grant agency: IGA MZ NC7548
Investigator: RNDr. Martina Saláková, Ph.d., Institution of hematology and blood transfusion
Keywords: TTV, prevalence, antibodies
Annotation: In the previous study the prevalence of TTV has been found by PCR in groups of blood donors and children and in groups of parental, transplacental and sexual risk of transmission. The rate of the detection of TTV DNA by PCR is influenced by the genomic areas, which are selected for primer annealing. The methods to detect anti TTV antibodies are required for completing epidemiology of TTV. The aim of this project is as follows: 1) to optimize the detection system for anti-TTV antibodies 2) to find the prevalence of TTV antibodies in population and 3) to compare the prevalence of TTV DNA and TTV antibodies.